In recent years, physical literacy has attracted international attention and due to its relevance to education and sports policy, much investment has been made in this model in different countries. Despite the importance of the issue of physical literacy, since this concept is in the early stages, there are still no precise instrument for evaluating it at different ages and between different groups. The aim of this study was to determine the validity and reliability of the Persian version of the Perceived Physical Literacy Instrument (PPLI) in physical education teachers. For this purpose, 208 male and female physical education teachers with an age range of 20 to 66 years completed questionnaire. First, the translation of the Persian version of the questionnaire was confirmed using the open translation method and then to determine the construct validity of the questionnaire, confirmatory factor analysis based on structural equation modeling and to determine internal consistency, Cronbach's alpha coefficient was used. Also, for time reliability of the questions, intra-class correlation with test-retest method was used. The results showed that the good fit index and the comparative fit index are higher than 0/9. Cronbach's alpha coefficient for perceived physical literacy and its dimensions above 0.7 (acceptable index value) were obtained. Based on the results, the Persian version of the Perceived Physical Literacy Instrument (PPLI) in physical education teachers has a constructive validity and internal and temporal reliability and can be used as a valid and reliable tool to assess the physical literacy of physical education teachers.