نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیات علمی/دانشگاه زنجان

2 عضو هیات علمی/ انستیتو پاستور ایران

3 عضو هیات علمی/ دانشگاه زنجان

چکیده

هدف از این پژوهش، تعیین تأثیر تجویز صفاقی انسولین بر عملکرد حرکتی موش­های صحرایی در ماز آبی می‌باشد. برای این منظور، تعداد 40 سر رت­ نر از نژاد آلبینو ویستار به‌صورت تصادفی در چهار گروه آزمایشی قرار گرفتند (هر گروه 10 سر رت). تزریق انسولین در چهار دوز (صفر، یک، شش و 12 واحد برکیلوگرم) و درطول یک هفته (روزانه یک‌بار) انجام شد. آموزش و آزمون حیوانات با استفاده از ماز آبی موریس صورت گرفت. جهت تحلیل آماری، آزمون تحلیل واریانس مختلط بین گروهی و درون‌گروهی (SPANOVA) به‌کار رفت. یافته­ها نشان داد که در مرحلۀ اکتساب، اثر اصلی زمانبرای مسافت طی شده و زمان تأخیر معنادار است .(P<0.001) همچنین، تزریق صفاقی انسولین بدون تأثیر معنادار بر سطوح گلوکز سرم در تمامی دوزها موجب کاهش مسافت طی‌شده (P<0.001) و به‌صورت وابسته به دوز، باعث کاهش زمان تأخیر (P=0.004) در مرحلۀ اکتساب شد. در آزمون یادداری نیز انسولین به‌صورت وابسته به دوز (یک و 12 واحد بر کیلوگرم) موجب کاهش زمان تأخیر در رسیدن به سکوی پنهان گردید. در‌نتیجه، تجویز محیطی انسولین دارای اثرات مرکزی بوده و می­تواند موجب ارتقای عملکرد حرکتی از طریق بهبود حافظه و یادگیری فضایی شود. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of systemic administration of insulin on spatial memory and learning in male rats

نویسندگان [English]

  • ahmad rahmani 1
  • Naser Naghdi 2
  • Javad Salehi 1
  • Ali Gorzi 3

چکیده [English]

Thus the aim of the current study was to determine whether intraperitoneal (i.p.) administration of insulin improves the water maze motor performance of the rats. Forty male wistar rats were randomly assigned to for groups (each 10 rats). Insulin was injected (0, 1, 6, and 12 U/Kg) once daily for one week. Animals training and testing were conducted using the Morris water maze. Data analyzed using split plot ANOVA (SPANOVA). Results showed significant main effect of time for distance and escape latency in acquisition stage (P<0.001). Also, intraperitoneal injection of insulin, without any significant effect on serum glucose levels, significantly reduced distance moved (P<0.001) and escape latency (P=0.004). Moreover, in retention test, insulin reduced escape latency to hidden platform in a dose dependent manner (dose 12 & 1 U/Kg). Therefore, peripheral administration of insulin has central effects and can enhance motor performance via improvement of spatial learning and memory. 

کلیدواژه‌ها [English]

  • Insulin
  • Motor performance
  • Spatial learning
  • Memory
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