مقایسة تأثیر استرس محدودیت حرکتی بر یادگیری و حافظة فضایی رت‌های نر پیر و جوان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی‌ارشد رفتار حرکتی، دانشگاه اصفهان

2 استادیار رفتارحرکتی، دانشگاه اصفهان

3 استاد فیزیولوژی، دانشگاه علوم پزشکی اصفهان

چکیده

هدف پژوهش حاضر، بررسی تأثیر استرس محدودیت حرکتی بر یادگیری و حافظة­ فضایی رت­های نر پیر و جوان بود. تعداد 24 رت نر نژاد ویستار از پژوهشکدة رویان اصفهان در دو گروه سنی سه‌ماهه (جوان) و 12 ماهه (پیر) انتخاب شدند و براساس وزن در چهار گروه شش‌تایی تقسیم شدند. پس از یک هفته سازگاری با محیط لانه و تردمیل، هر گروه با توجه به برنامة­ خاص خود شامل، گروه استرس محدودیت حرکتی (روزی دو ساعت به‌مدت هفت روز) و گروه کنترل به تمرین پرداختند. برای اندازه­گیری میزان یادگیری و حافظة­ فضایی از آزمون مازآبی موریس استفاده شد. تجزیه‌وتحلیل داده­ها با استفاده از تحلیل واریانس دوطرفه انجام شد. نتایج نشان داد که استرس محدودیت حرکتی، یادگیری رت­های پیر و جوان را تخریب می‌کند؛ اما بر حافظة­ فضایی آن‌ها اثر معناداری برجای نمی‌گذارد؛ به‌طوری‌که یادگیری رت­های جوان دراثر استرس در مقایسه با رت ­های پیر کمتر تخریب می‌شود. نتایج حاصل از پژوهش به تأثیر منفی استرس محدودیت حرکتی به‌ویژه در یادگیری اشاره دارد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The Comparison of Immobilization Stress on Learning and Spatial Memory in Young and Old Male Rats

نویسندگان [English]

  • Roya Mirzarazi Dehaghi 1
  • Shila Safavi Homami 2
  • Hojjatollah Alaei 3
1 M.Sc. of Motor Behavior, University of Isfahan
2 Assistant Professor of Motor Behavior, University of Isfahan
3 Professor of Physiology, Isfahan University of Medical Sciences
چکیده [English]

The main purpose of this study was to investigate the effects of immobilization stress on learning and spatial memory between young and old male rats. In order 24 male Wistar rats were selected from Royan institute of Isfahan and divided into 2 age groups based on their weights (3 months= young and 12 months= old). After one week of adaptation to the nest environment, each group (4 groups, n=6) takes part in their special program contains immobility stress group (2h×7days), and sham group. Learning and spatial memory measured by using Morris water maze. Data were analyzed by using independent T-test and two-way variance analyses test. Results showed that immobility stress destroyed learning in young and old rats but had no effect on spatial memory and learning of young rats was less damaged by stress than old rats. The results of this research confirmed the negative effect of immobility stress, especially in learning.

کلیدواژه‌ها [English]

  • Immobilization Stress
  • Learning
  • Spatial Memory
  • Age
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